Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Identifieur interne : 001829 ( Main/Exploration ); précédent : 001828; suivant : 001830Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.
Auteurs : Jasper Fuk-Woo Chan [République populaire de Chine] ; Garnet Kwan-Yue Choi [République populaire de Chine] ; Alan Ka-Lun Tsang [République populaire de Chine] ; Kah-Meng Tee [République populaire de Chine] ; Ho-Yin Lam [République populaire de Chine] ; Cyril Chik-Yan Yip [République populaire de Chine] ; Kelvin Kai-Wang To [République populaire de Chine] ; Vincent Chi-Chung Cheng [République populaire de Chine] ; Man-Lung Yeung [République populaire de Chine] ; Susanna Kar-Pui Lau [République populaire de Chine] ; Patrick Chiu-Yat Woo [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; Bone Siu-Fai Tang [République populaire de Chine] ; Kwok-Yung Yuen [Hong Kong]Source :
- Journal of clinical microbiology [ 1098-660X ] ; 2015.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Jeune adulte, Mâle, Nourrisson, Oligonucléotides (génétique), RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Sondes oligonucléotidiques (génétique), Sujet âgé, Sujet âgé de 80 ans ou plus.
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus, Oligonucléotides, Sondes oligonucléotidiques.
- isolement et purification : Coronavirus.
- virologie : Infections à coronavirus.
- Adolescent, Adulte, Adulte d'âge moyen, Animaux, Coronavirus, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Nourrisson, RT-PCR, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Sujet âgé, Sujet âgé de 80 ans ou plus.
English descriptors
- KwdEn :
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Female, Humans, Infant, Male, Middle Aged, Oligonucleotide Probes (genetics), Oligonucleotides (genetics), Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity, Young Adult.
- MESH :
- chemical , genetics : Oligonucleotide Probes, Oligonucleotides.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Adolescent, Adult, Aged, Aged, 80 and over, Animals, Child, Child, Preschool, Female, Humans, Infant, Male, Middle Aged, Sensitivity and Specificity, Young Adult.
Abstract
Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.
DOI: 10.1128/JCM.01224-15
PubMed: 26019210
Affiliations:
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Le document en format XML
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Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Garnet Kwan Yue" sort="Choi, Garnet Kwan Yue" uniqKey="Choi G" first="Garnet Kwan-Yue" last="Choi">Garnet Kwan-Yue Choi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tee, Kah Meng" sort="Tee, Kah Meng" uniqKey="Tee K" first="Kah-Meng" last="Tee">Kah-Meng Tee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Lam, Ho Yin" sort="Lam, Ho Yin" uniqKey="Lam H" first="Ho-Yin" last="Lam">Ho-Yin Lam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</nlm:affiliation><country wicri:rule="url">Hong Kong</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26019210</idno><idno type="pmid">26019210</idno><idno type="doi">10.1128/JCM.01224-15</idno><idno type="wicri:Area/PubMed/Corpus">001614</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001614</idno><idno type="wicri:Area/PubMed/Curation">001614</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001614</idno><idno type="wicri:Area/PubMed/Checkpoint">001578</idno><idno 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University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Choi, Garnet Kwan Yue" sort="Choi, Garnet Kwan Yue" uniqKey="Choi G" first="Garnet Kwan-Yue" last="Choi">Garnet Kwan-Yue Choi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tee, Kah Meng" sort="Tee, Kah Meng" uniqKey="Tee K" first="Kah-Meng" last="Tee">Kah-Meng Tee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Lam, Ho Yin" sort="Lam, Ho Yin" uniqKey="Lam H" first="Ho-Yin" last="Lam">Ho-Yin Lam</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathology, Hong Kong Sanatorium and Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region, China kyyuen@hku.hk.</nlm:affiliation><country wicri:rule="url">Hong Kong</country><wicri:regionArea>State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong Special Administrative Region, China Department of Microbiology, The University of Hong Kong, Hong Kong Special Administrative Region, China Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong Special Administrative Region, China Carol Yu Centre for Infection, The University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Oligonucleotide Probes (genetics)</term><term>Oligonucleotides (genetics)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>Oligonucléotides (génétique)</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Sondes oligonucléotidiques (génétique)</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Oligonucleotide Probes</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term><term>Oligonucléotides</term><term>Sondes oligonucléotidiques</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Adult</term><term>Aged</term><term>Aged, 80 and over</term><term>Animals</term><term>Child</term><term>Child, Preschool</term><term>Female</term><term>Humans</term><term>Infant</term><term>Male</term><term>Middle Aged</term><term>Sensitivity and Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adolescent</term><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Coronavirus</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Nourrisson</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Sujet âgé</term><term>Sujet âgé de 80 ans ou plus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses. </div></front></TEI><affiliations><list><country><li>Hong Kong</li><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chan, Jasper Fuk Woo" sort="Chan, Jasper Fuk Woo" uniqKey="Chan J" first="Jasper Fuk-Woo" last="Chan">Jasper Fuk-Woo Chan</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Cheng, Vincent Chi Chung" sort="Cheng, Vincent Chi Chung" uniqKey="Cheng V" first="Vincent Chi-Chung" last="Cheng">Vincent Chi-Chung Cheng</name><name sortKey="Choi, Garnet Kwan Yue" sort="Choi, Garnet Kwan Yue" uniqKey="Choi G" first="Garnet Kwan-Yue" last="Choi">Garnet Kwan-Yue Choi</name><name sortKey="Lam, Ho Yin" sort="Lam, Ho Yin" uniqKey="Lam H" first="Ho-Yin" last="Lam">Ho-Yin Lam</name><name sortKey="Lau, Susanna Kar Pui" sort="Lau, Susanna Kar Pui" uniqKey="Lau S" first="Susanna Kar-Pui" last="Lau">Susanna Kar-Pui Lau</name><name sortKey="Tang, Bone Siu Fai" sort="Tang, Bone Siu Fai" uniqKey="Tang B" first="Bone Siu-Fai" last="Tang">Bone Siu-Fai Tang</name><name sortKey="Tee, Kah Meng" sort="Tee, Kah Meng" uniqKey="Tee K" first="Kah-Meng" last="Tee">Kah-Meng Tee</name><name sortKey="To, Kelvin Kai Wang" sort="To, Kelvin Kai Wang" uniqKey="To K" first="Kelvin Kai-Wang" last="To">Kelvin Kai-Wang To</name><name sortKey="Tsang, Alan Ka Lun" sort="Tsang, Alan Ka Lun" uniqKey="Tsang A" first="Alan Ka-Lun" last="Tsang">Alan Ka-Lun Tsang</name><name sortKey="Woo, Patrick Chiu Yat" sort="Woo, Patrick Chiu Yat" uniqKey="Woo P" first="Patrick Chiu-Yat" last="Woo">Patrick Chiu-Yat Woo</name><name sortKey="Yeung, Man Lung" sort="Yeung, Man Lung" uniqKey="Yeung M" first="Man-Lung" last="Yeung">Man-Lung Yeung</name><name sortKey="Yip, Cyril Chik Yan" sort="Yip, Cyril Chik Yan" uniqKey="Yip C" first="Cyril Chik-Yan" last="Yip">Cyril Chik-Yan Yip</name></country><country name="Hong Kong"><noRegion><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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